United Kingdom Information Product Development Technical Reference Sugar Beet Pests and Diseases Beet Cyst Nematode Beet Cyst Nematode Sampling

Beet Cyst Nematode Sampling

Soil Sampling Protocol

This protocol provides some general guidelines and advice on method, frequency and timing of sampling soil for beet cyst nematode.


1. Field sampling: – In order to take a representative and random sample from a field in a practical manner, the ’W’ – pattern is recommended to be used across the field (see image).

2. Sampler type: – The best tool for taking samples is to use a cheese corer (see photo) however a small trowel or similar tool can be used. The ideal sampling depth is 20cm (ploughing depth).

3. Timing: – Soils can be sampled after cereal harvest and prior to beet drilling, allowing enough time for samples to be sent away and test results received whereupon a decision on variety choice can be made. 

4. Number of cores & quantity of soil: – For each hectare, it is recommended to take a minimum of 20 separate soil cores. For example, an area of 8 ha should produce at least 80 soil cores. It is worth to split fields bigger than 20 ha into separate sections for testing.  In general, the higher the number of subsamples the more accurate the test result.

5. Soil Preparation: The collected soil should be thoroughly but gently mixed or damage to cyst can occur! Around 800-1000g of soil should be extracted and placed in a labelled polythene bag.

6. Storage and Packaging: – Where samples cannot be sent immediately they should be stored temporarily in cool (<10˚ C), dry conditions or a hatch of larvae is likely and nematode population cannot be ascertained precisely. Samples should be sealed in strong polythene bags. The bags should be securely sealed and must be labelled clearly so the contact person, address, phone no, email and the field of each sample can be easily identified.

7. Sending sample to lab: – Beet cyst nematodes are relatively fragile and can be destroyed by rough handling. Posting is fine provided that some protective packaging is used such as a cardboard box or bubble wrap. To get an accurate test result the sample should arrive at the lab as soon as possible and therefore a courier service is recommended.

 

All samples should be addressed and sent to:

Simon Witheford

KWS UK LTD

Thriplow Nr. Royston

Herts SG8 7RE

United Kingdom

 

For further information contact:

Simon Witheford

KWS UK Ltd.

Product Manager - Sugar Beet

 

Direct: 01763 207304

Mobile: 07717 844441

simon.witheford@kws-uk.com